Technological and economic analysis of electrokinetic remediation of contaminated soil: A global perspective and its application in Indian scenario.

Globally million hectares of land annually is getting contaminated by heavy metalloids like As, Cd, Cr, Hg, Pb, Co, Cu, Ni, Zn, and Se, with current concentrations in soil above geo-baseline or regulatory standards. The heavy metals are highly toxic, mobile, and persistent and hence require immediate and effective mitigation. There are many available remediation techniques like surface capping, encapsulation, landfilling, soil flushing, soil washing, electrokinetic extraction, stabilization, solidification, vitrification, phytoremediation, and bioremediation which have been evolved to clean up heavy metal-contaminated sites. Nevertheless, all of the technologies have some applicability and limitations making the soil remediation initiative unsustainable. Among the available technologies, electrokinetic remediation (EKR) has been comparatively recognized to mitigate contaminated sites via both in-situ and ex-situ approaches due to its efficiency, suitability for use in low permeability soil, and requirement of low potential gradient. The work critically analyzes the EKR concerning techno, economic, and sustainability aspect for evaluating its application on various substrates and environmental conditions. The current soil contamination status in India is presented and the application of EKR for the heavy metal remediation from soil has been evaluated. The present work summaries a comprehensive and exhaustive review on EKR technology proving its effectiveness for a country like India where the huge amount of waste generated could not be treated due to lack of infrastructure, technology, and economic constraints.

Envisioning the innovative approaches to achieve circular economy in the water and wastewater sector.

Given the challenges of urbanization and rapid resource depletion, policymakers have been compelled to abandon the old sequential paradigm of "take-make-use-dispose" to a circular approach that prioritizes preservation of natural resources. The circular economy represents a sustainable management concept that focuses on reducing, recovering, reusing, and recycling waste. While significant strides have been made in implementing circular economy principles in various industries such as automotive, electronics, and construction, particular attention has been given to the water and wastewater domains due to imbalances in water resources. Here we review the global progress of circular economy adoptability in the water and wastewater domains, considering technical, environmental, economic, and social perspectives. It assesses the current state of circular economy integration in the wastewater domain worldwide and presents approaches to promote and accelerate its adoption. The study critically examines the principles of waste management, known as the 6Rs (reclaim, restore, recycle, reduce, recover, reuse), in order to formulate effective strategies for integrating circular economy practices in the water and wastewater domains. Additionally, the study provides an overview of existing research conducted on different aspects of circular economy. Finally, the study analyzes the challenges and opportunities associated with implementing circular economy principles in the water sector.

Waste coconut shell-derived carbon monolith as an efficient binder-free cathode for electrochemical advanced oxidation treatment of endocrine-disrupting compounds.

Discharge of endocrine-disrupting compounds such as methylparaben (MePa) into natural water bodies deteriorates the aquatic ecosystem. In this regard, electrochemical oxidation (EO) and electro-Fenton (EF) processes are acknowledged as effective methods to eliminate biorecalcitrant compounds from different wastewater matrices. In these systems, the H2O2-producing ability of carbon-based cathodes is put to advantage for producing homogenous hydroxyl radicals by simulating Fenton's reaction, which dramatically augments the contaminant removal efficiency. However, commercial carbon based cathodes are not economically affordable, especially for voluminous treatment. Hence in the present work, waste-derived carbonised coconut shell (CCS) monolith was employed as a cathode in EO and EF treatment of MePa. Almost the entire MePa with initial concentration of 10 mg/L was removed in 60 min by EO and 45 min by EF process at neutral pH, applied current density of 7.5 mA/cm2, NaCl concentration of 1.0 g/L and 10 mg/L of Fe2O3 dosing. The MePa removal efficiency of the CCS cathode-fitted system after 60 min was better than the commercial graphite plate and Ti-based mixed metal oxide employing system due to higher H2O2 electrosynthesis (H2O2 = 9.0 ± 0.6 mg/L after 60 min). Moreover, the same setup was used for treating 10 mg/L of MePa-spiked real sewage and demonstrated MePa and total organic carbon removal efficiency of 80.16 ± 2.31% and 37.42 ± 3.50%, respectively, in 45 min. Further, the CCS-mediated EF treatment achieved >90% removal of MePa for eight continuous batch cycles and recorded a current density drop of just 0.23% per cycle. The degradation pathway and toxicity assessment of the intermediates using the Ecological Structure Activity Relationships (ECOSAR) tool supported the eco-friendliness of the current treatment scheme.

Nickel-iron-driven heterogenous bio-electro-fenton process for the degradation of methylparaben.

Discharge of emerging contaminants such as parabens in natural water bodies is a grievous concern. Among parabens, methylparaben (MP) is most prevalent due to its extensive usage in personal care and food products and has been purported to trigger hormonal-related diseases. In this regard, the bio-electro-Fenton (BEF) process garners attention for remediating refractory compounds because of its ability to generate in situ hydroxyl radicals (•OH) utilising the energy harvested from electroactive microorganisms. In the present investigation, a Ni-Fe-driven heterogenous BEF system (BEF-MFC) was used to degrade MP from different matrices. At neutral catholyte pH, 99.54 ± 0.22% of MP was removed from an initial concentration of 10 mg/L in 240 min of retention time with an estimated treatment cost of about 1.01 $/m3. The removal rate ameliorated when the catholyte pH was dropped to 3.0 and by imposing an external voltage of 0.5 V, requiring just 120 min to achieve comparable MP removal efficiencies. However, catalyst leaching was higher at acidic pH (leaching of Fe ions = 0.44 mg/L and Ni ions = 0.06 mg/L) and applying external voltage increased the treatment cost slightly to 1.08 $/m3. Further, treatment of 10 mg/L MP-spiked real wastewater at pH of 7.0 with the BEF-MFC attained 85.70 ± 3.30% and 56.50 ± 1.70% reduction in MP and total organic carbon, respectively, in 240 min. In addition, a maximum power density of 205.90 ± 2.27 mW/cm2 was harvested in the BEF-MFC; thus, portraying the dual benefit of Ni-Fe heterogeneous catalyst. Even though, Ni-Fe performed reasonably well as Fenton-cum-cathode catalyst, future endeavours should be poised to fine-tune catalysts to accelerate H2O2 and •OH generation, which will reinforce the scalability of this system.

Role of bacterial quorum sensing and quenching mechanism in the efficient operation of microbial electrochemical technologies: A state-of-the-art review.

Microbial electrochemical technologies (METs) are a group of innovative technologies that produce valuables like bioelectricity and biofuels with the simultaneous treatment of wastewater from microorganisms known as electroactive microorganisms. The electroactive microorganisms are capable of transferring electrons to the anode of a MET through various metabolic pathways such as direct (via cytochrome or pili) or indirect (through transporters) transfer. Though this technology is promising, the inferior yield of valuables and the high cost of reactor fabrication are presently impeding the large-scale application of this technology. Therefore, to overcome these major bottlenecks, a lot of research has been dedicated to the application of bacterial signalling, for instance, quorum sensing (QS) and quorum quenching (QQ) mechanisms in METs to improve its efficacy in order to achieve a higher power density and to make it more cost-effective. The QS circuit in bacteria produces auto-inducer signal molecules, which enhances the biofilm-forming ability and regulates the bacterial attachment on the electrode of METs. On the other hand, the QQ circuit can effectively function as an antifouling agent for the membranes used in METs and microbial membrane bioreactors, which is imperative for their stable long-term operation. This state-of-the-art review thus distinctly describes in detail the interaction between the QQ and QS systems in bacteria employed in METs to generate value-added by-products, antifouling strategies, and the recent applications of the signalling mechanisms in METs to improve their yield. Further, the article also throws some light on the recent advancements and the challenges faced while incorporating QS and QQ mechanisms in various types of METs. Thus, this review article will help budding researchers in upscaling METs with the integration of the QS signalling mechanism in METs.

Appraising efficacy of existing and advanced technologies for the remediation of beta-blockers from wastewater: A review.

The discharge of emerging pollutants, such as beta-blockers (BB), has been recognized as one of the major threats to the environment due to the ecotoxicity associated with these emerging pollutants. The BB are prescribed to treat high blood pressure and cardiovascular diseases; however, even at lower concentration, these pollutants can pose eco-toxic impacts towards aquatic organisms. Additionally, owing to their recalcitrant nature, BB are not effectively removed through conventional technologies, such as activated sludge process, trickling filter and moving bed bioreactor; thus, it is essential to understand the degradation mechanism of BB in established as well as embryonic technologies, like adsorption, electro-oxidation, Fenton process, ultraviolet-based advance oxidation process, ozonation, membrane systems, wetlands and algal treatment. In this regard, this review articulates the recalcitrant nature of BB and their associated removal technologies. Moreover, the major advantages and limitations of these BB removal technologies along with the recent advancements with regard to the application of innovative materials and strategies have also been elucidated. Therefore, the present review intends to aid the researchers in improving the BB removal efficiency of these technologies, thus alleviating the problem of the release of BB into the environment.

Evaluating application of photosynthetic microbial fuel cell to exhibit efficient carbon sequestration with concomitant value-added product recovery from wastewater: A review.

The emission of CO2 from industrial (24%) and different anthropogenic activities, like transportation (27%), electricity production (25%), and agriculture (11%), can lead to global warming, which in the long term can trigger substantial climate changes. In this regard, CO2 sequestration and wastewater treatment in tandem with bioenergy production through photosynthetic microbial fuel cell (PMFC) is an economical and sustainable intervention to address the problem of global warming and elevating energy demands. Therefore, this review focuses on the application of different PMFC as a bio-refinery approach to produce biofuels and power generation accompanied with the holistic treatment of wastewater. Moreover, CO2 bio-fixation and electron transfer mechanism of different photosynthetic microbiota, and factors affecting the performance of PMFC with technical feasibility and drawbacks are also elucidated in this review. Also, low-cost approaches such as utilization of bio-membrane like coconut shell, microbial growth enhancement by extracellular cell signalling mechanisms, and exploitation of genetically engineered strain towards the commercialization of PMFC are highlighted. Thus, the present review intends to guide the budding researchers in developing more cost-effective and sustainable PMFCs, which could lead towards the commercialization of this inventive technology.

Are integrated bioelectrochemical technologies feasible for wastewater management?

The need for sustainable technological solutions for wastewater management at different scales has led to the emergence of several promising integrated bioelectrochemical technologies in the past decade. A thorough assessment of these technologies is imperative to understand their practical implementation feasibility and to identify the key challenges to prioritise the research and development work. Our multicriteria-based assessment reveals that the integrated technologies are efficient for wastewater treatment in terms of normalised land footprint [(0.31-1.39 m2/population equivalent (PE))] - and energy consumption (0.18-1.49 kWH/m3) as compared to the conventional biotechnologies, and suggests that they have potential for real-world application. Specifying the boundaries according to their treatment capabilities and scale-up potential besides niche application sites or geographical locations is required to expedite their transition to the real-world wastewater management sector.

Enzymatic cell disruption followed by application of imposed potential for enhanced lipid extraction from wet algal biomass employing photosynthetic microbial fuel cell.

Solvent-free algal cell lysis using fungal enzyme for enhanced lipid recovery diminishes per unit production cost of algal biodiesel. In this investigation, a triple chamber photosynthetic microbial fuel cell (PMFC) was fabricated, where positive potential was imposed in the extraction chamber to draw the negatively charged lipid ions from the cathodic chamber. Under optimum imposed potential of + 3.0 V (vs standard hydrogen electrode) and with 3.5% (v/v) dosage of fungal enzyme in to the algal consortium of cathodic chamber, a maximum of 79.0% of lipid was recovered. Additionally, enzyme-assisted de-oiled algal biomass was applied in the anodic chamber to function as substrate and mediator for exo-electrogens, and the maximum power density of 10.0 W/m3 with 82.4% removal of chemical oxygen demand was achieved while treating synthetic wastewater. Therefore, this cost-effective exploration demonstrated successful bioelectricity production and concomitant wastewater treatment with solvent-free direct lipid recovery from wet algal biomass through PMFC.

Efficacious bioremediation of heavy metals and radionuclides from wastewater employing aquatic macro- and microphytes.

Cytotoxic, mutagenic, and carcinogenic contaminants, such as heavy metals and radionuclides, have become an alarming environmental concern globally, especially for developed and developing nations. Moreover, inefficient prevalent wastewater treatment technologies combined with increased industrial activity and modernization has led to increase in the concentration of toxic metals and radioactive components in the natural water bodies. However, for the improvement of ecosystem of rivers, lakes, and other water sources different physicochemical methods such as membrane filtration, reverse osmosis, activated carbon adsorption, electrocoagulation, and other electrochemical treatment are employed, which are uneconomical and insufficient for the complete abatement of these emerging pollutants. Therefore, the application of bioremediation employing aquatic macrophytes and microphytes have gained considerable importance owing to the benefits of cost-effectiveness, eco-friendly, and higher energy efficiency. Thus, the present review aims to enlighten the readers on the potential application of algae, cyanobacteria, plant, and other aquatic micro- and macrophytes for the elimination of carcinogenic metals and radioactive isotopes from wastewater. Additionally, the use of transgenic plants, genetically modified species, algal-bacterial symbiosis for the enhancement of removal efficiency of mutagenic contaminants are also highlighted. Furthermore, species selection based on robustness, mechanism of different pathways for heavy metal and radionuclide detoxification are elucidated in this review article.

Bacterial signalling mechanism: An innovative microbial intervention with multifaceted applications in microbial electrochemical technologies: A review.

Microbial electrochemical technologies (METs) are a set of inventive tools that generate value-added by-products with concomitant wastewater remediation. However, due to the bottlenecks, like higher fabrication cost and inferior yield of resources, these inventive METs are still devoid of successful field-scale implementation. In this regard, application of quorum sensing (QS) mechanism to improve the power generation of the METs has gained adequate attention. The QS is an intercellular signalling mechanism that controls the bacterial social network in its vicinity via the synthesis of diffusible signal molecules labelled as auto inducers, thus ameliorating yield of valuables produced through METs. This state-of-the-art review elucidates different types of QS molecules and their working mechanism with the special focus on the widespread application of QS in the field of METs for their performance enhancement. Thus, this review intends to guide the researchers in rendering scalability to METs by integrating innovative QS mechanisms into them.

Role of bioelectrochemical systems for the remediation of emerging contaminants from wastewater: A review.

Bioelectrochemical systems (BESs) are a unique group of wastewater remediating technology that possesses the added advantage of valuable recovery with concomitant wastewater treatment. Moreover, due to the application of robust microbial biocatalysts in BESs, effective removal of emerging contaminants (ECs) can be accomplished in these BESs. Thus, this review emphasizes the recent demonstrations pertaining to the removal of complex organic pollutants of emerging concern present in wastewater through BES. Owing to the recalcitrant nature of these pollutants, they are not effectively removed through conventional wastewater treatment systems and thereby are discharged into the environment without proper treatment. Application of BES in terms of ECs removal and degradation mechanism along with valuables that can be recovered are discussed. Moreover, the factors affecting the performance of BES, like biocatalyst, substrate, salinity, and applied potential are also summarized. In addition, the present review also elucidates the occurrence and toxic nature of ECs as well as future recommendations pertaining to the commercialization of this BES technology for the removal of ECs from wastewater. Therefore, the present review intends to aid the researchers in developing more efficient BESs for the removal of ECs from wastewater.

Application of microbial electrochemical technologies for the treatment of petrochemical wastewater with concomitant valuable recovery: A review.

Petrochemical industry is one of the major and rapidly growing industry that generates a variety of toxic and recalcitrant organic pollutants as by-products, which are not only harmful to the aquatic animals but also affects human health. The majority of the components of petrochemical wastewater (PW) are carcinogenic, genotoxic and phytotoxic in nature; hence, this complex wastewater generated from different petrochemical processes should be efficiently treated prior to its disposal in natural water bodies. The established technologies like advanced oxidation, membrane bioreactor, electrocoagulation and activated sludge process employed for the treatment of PW are highly energy intensive and incurs high capital and operation cost. Moreover, these technologies are not effective in completely eliminating petroleum hydrocarbons present in PW. Thus, to reduce the energy requirement and also to transform the chemical energy trapped in these organic matters present in this wastewater into bioelectricity and other value-added products, microbial electrochemical technologies (METs) can be efficaciously used, which would also compensate the treatment cost by transforming these pollutants into bioenergy and valuables. In this regard, this review elucidates the feasibility and application of different METs as an appropriate alternative for the treatment of PW. Furthermore, the numerous bottlenecks towards the real-life application and commercialization of pioneering METs have also been articulated.

Live diatoms as potential biocatalyst in a microbial fuel cell for harvesting continuous diafuel, carotenoids and bioelectricity.

Microbial fuel cell (MFC) with live diatoms (Nitzschia palea) displacing bacteria in the anodic chamber generated electrical potential. Unlike other microalgae, diatoms fix 25% of atmospheric CO2, thus releasing O2. They perform photolysis of water by photosynthesis in the plastid during light photoperiod and cellular respiration in the mitochondria during dark, producing electrons and protons, respectively. The electrogenic property of diatom was explored and evaluated by comparing the potential changes with reference fuel cell without diatoms and that operated with diatoms in the anodic chamber. Such photosynthetic diatom microbial fuel cell (PDMFC) employed f/2 media rich in nitrates, phosphates, metasilicates, trace metals and vitamins as the anolyte and potassium permanganate as catholyte enhanced the output voltage by 3rd day. The maximum power density for PDMFC was 12.62 mWm-2 and coulombic efficiency of 22.95%. Besides this, the fixed diatom cells at anode showed about 64.28% increase in lipid production on 15th day compared to that on 1st day along with the increment in formation of complex fatty acid methyl esters and carotenoids during its operation. Hence, diatoms can be envisaged to substitute bacteria in the anodic chamber of MFC to simultaneously produce bioelectricity and other valuable compounds. Further their silica nanoporous architecture serve as good absorbents for heavy metal removal found in many wastewaters.

High throughput techniques for the rapid identification of electroactive microorganisms.

Electroactive microorganisms (EAM), capable of executing extracellular electron transfer (EET) in/out of a cell, are employed in microbial electrochemical technologies (MET) and bioelectronics for harnessing electricity from wastewater, bioremediation and as biosensors. Thus, investigation on EAM is becoming a topic of interest for multidisciplinary areas, such as environmental science, energy and health sectors. Though, EAM are widespread in three domains of life, nevertheless, only a few hundred EAM have been identified so far and hence, the rapid identification of EAM is imperative. In this review, the techniques that are developed for the direct identification of EAM, such as azo dye and WO3 based techniques, dielectrophoresis, potentiostatic/galvanometric techniques, and other indirect methods, such as spectroscopy and molecular biology techniques, are highlighted with a special focus on time required for the detection of these EAM. The bottlenecks for identifying EAM and the knowledge gaps based on the present investigations are also discussed. Thus, this review is intended to encourage researchers for devolving high-throughput techniques for identifying EAM with more accuracy, while consuming less time.

Tungsten oxide as electrocatalyst for improved power generation and wastewater treatment in microbial fuel cell.

Microbial fuel cell (MFC) is a device that oxidizes the organic matter present in wastewater and simultaneously generates electricity from it. For practical applications, the power production of MFCs needs to be enhanced and the use of novel anode and cathode catalyst can certainly help in this regard. Such a novel catalyst, WO3, was explored as both anode and cathode catalyst in this study. Performance of MFCs was enhanced when WO3 was used as an electrocatalyst. The maximum power density of MFC was increased by five times when WO3 was used as anode catalyst and by four times when it was used as cathode catalyst as compared to control MFC using electrode without any catalyst. Almost six times increment in maximum power production of MFC was observed when WO3 was used as catalyst on both the electrodes. Electrochemical analysis of WO3 also proved that it could enhance the current density of the modified electrode owing to its electrochemical catalytic properties. Furthermore, chemical oxygen demand (COD) removal of MFC having WO3 coated electrodes was also observed to be higher, thus suggesting an overall enhancement in the performance of MFC by the use of WO3 as an electrocatalyst.

Quorum-sensing mediated signals: A promising multi-functional modulators for separately enhancing algal yield and power generation in microbial fuel cell.

Quorum-sensing molecules (QSMs) extracted from anaerobic sludge can help to enhance the overall productivity of algal culture, thus diminishing the per unit production cost of algae based-biofuel. In this investigation, QSMs extracted from anaerobic bacterial sludge of microbial fuel cell (MFC) was used to enhance the overall productivity of Chlorella sorokiniana cultivated in a separate bubble column photobioreactor. With the dosage of QSMs, algal biomass productivity and lipid content were increased by 2.25 times and 1.28 times, respectively. Further, lipid extracted biomass of quorum-sensing induced algae (LEB-QSA) was applied in anodic chamber of MFC to function as substrate and mediator, which enhanced the coulombic efficiency of this MFC by 74% as compared to the control MFC operated without LEB-QSA. Thus, this exploration demonstrated successful improvement in the macromolecular properties of algal culture dosed with QSMs and improved performance of MFC with the application of LEB-QSA as mediator and substrate.

Increasing methane content in biogas and simultaneous value added product recovery using microbial electrosynthesis.

Electrosynthesis of multi-carbon compounds from the carbon dioxide present in biogas is a nascent approach towards purification of biogas. Microbial electrosynthesis (MES) cells, fabricated using different electrode materials, were operated using different electrolytes and mixed anaerobic culture as biocatalysts in the cathodic chamber under an applied cathode potential of -0.7 V vs standard hydrogen electrode (SHE). The rate of production of acetate, isobutyrate, propionate and 2-piperidinone from reduction of CO2 in the cathodic chamber of the MES was 0.81 mM/day, 0.63 mM/day, 0.44 mM/day and 0.53 mM/day, respectively. As methane was also present in the biogas, methyl derivatives of these acids were also found in traces in catholyte. It was observed that the use of nickel foam as an anode, 1 M NiSO4 solution as anolyte, graphite felt as a cathode, phosphate buffer solution as catholyte at a pH of 5.2 proved to be the best possible combination for MES for this study to get enhanced product yield at higher energy efficiency.